mouse antibody  (Developmental Studies Hybridoma Bank)

Journal: bioRxiv

Article Title: Smoothened turnover regulated by Hedgehog signaling in Drosophila

doi: 10.64898/2026.01.08.698469

Figure Lengend Snippet: (A-C) Sagittal views of wing discs stained with (A,A’) α-Smo (gray), α-Ptc (green), and phalloidin (blue), or (B,B’) expressing BAC-GFP:Smo (green), BAC-Cherry:Smo (red), and mIFP ( ptc-Gal4>mIFP; Smo:GFP:Cherry/+ ); dashed lines indicate apical surface. (C-G) Wing disc expressing BAC-GFP:Smo (green) and Cad:Cherry; (C) sagittal, (D-G) frontal views in A compartment, P compartment and in apical or basolateral sections. (H-L) Same as (C-G), except wing discs express Cad:GFP (green) and BAC-encoded Cherry:Smo (red).

Article Snippet: The following antibodies were used: mouse α-GFP (Roche), rabbit α-RFP (Rockland), mouse α-Ptc (DSHB, Apa1), mouse α-En (DSHB, 4D9), DAPI, Phalloidin, mouse α-Dlg1, (DSHB, 4F3), α-Rab7 , α-Smo (DSHB, 20C6), Alexa633 conjugated-Phalloidin (Invitrogen), DAPI (Invitrogen), AbN ( ).

Techniques: Staining, Expressing

Journal: bioRxiv

Article Title: Smoothened turnover regulated by Hedgehog signaling in Drosophila

doi: 10.64898/2026.01.08.698469

Figure Lengend Snippet: Wild-type wing disc stained with α-Smo (red) and α-Ptc (green) at apical (A,A’) and basolateral (B,B’) optical planes. (A,B) Smo and Ptc staining; (A’,B’) Smo staining only. (A”,B”) Graphs showing intensity plots of images in (A,B) across A/P axis. (C,D) Same as (A,B) for unfixed disc expressing BAC-encoded GFP:Smo and mIFP driven by ptc-Gal4 showing fluorescence of Smo (green) and mIFP (blue) ( ptc-Gal4>mIFP ; GFP: Smo / + ). (E,F) Same as (C,D) for disc expressing BAC-encoded Cherry:Smo (red) and mIFP driven by ptc-Gal4 ( ptc-Gal4>mIFP ; Cherry : Smo / + ). (G) Same as (F) showing membrane-localized fluorescence only.

Article Snippet: The following antibodies were used: mouse α-GFP (Roche), rabbit α-RFP (Rockland), mouse α-Ptc (DSHB, Apa1), mouse α-En (DSHB, 4D9), DAPI, Phalloidin, mouse α-Dlg1, (DSHB, 4F3), α-Rab7 , α-Smo (DSHB, 20C6), Alexa633 conjugated-Phalloidin (Invitrogen), DAPI (Invitrogen), AbN ( ).

Techniques: Staining, Expressing, Fluorescence, Membrane

Journal: bioRxiv

Article Title: Smoothened turnover regulated by Hedgehog signaling in Drosophila

doi: 10.64898/2026.01.08.698469

Figure Lengend Snippet: (A-B’) Wing discs expressing mCD8:Cherry (red) by hh -Gal4 stained with α-Cut antibody to mark myoblasts (green). (A) notum region; (B) higher magnification image of boxed region in (A). Dashed white line encircles myoblasts. (C) Distribution of BAC encoded Hh:GFP (green) in the myoblasts (bounded by white dashed line; α-RFP, red). (D) Myoblasts encircled by white dashed line and marked by mCD8:Cherry (15B03Gal4>mCD8:Cherry; α-RFP, red) and α-Ptc antibody staining (green). (E-E”) Myoblasts (outlined with white dashed line and marked by expression of Cut, blue) expressing BAC encoded GFP:Cherry:Smo stained with α-GFP (green) and α-RFP (red) antibodies. (F) Clones induced as in expressing SmoRNAi (green) stained for Ptc (α-Ptc, red) bounded by white dashed line. Wild-type myoblasts are bounded by blue dashed line. (G-G”) Myoblasts (outlined with white dashed line and marked by expression of Mef2-Gal4>mIFP, blue) expressing BAC encoded GFP:Cherry:Smo (green) and α-RFP (red).

Article Snippet: The following antibodies were used: mouse α-GFP (Roche), rabbit α-RFP (Rockland), mouse α-Ptc (DSHB, Apa1), mouse α-En (DSHB, 4D9), DAPI, Phalloidin, mouse α-Dlg1, (DSHB, 4F3), α-Rab7 , α-Smo (DSHB, 20C6), Alexa633 conjugated-Phalloidin (Invitrogen), DAPI (Invitrogen), AbN ( ).

Techniques: Expressing, Staining, Clone Assay